Bright Anti-CD4

Charite Strasse 1
Deutsches Rheuma-Forschungszentrum
Research Scientist

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Company:

ThermoFisher Invitrogen

Product Name:

CD4 Monoclonal Antibody (RM4-5), PE-Cyanine7, eBioscience™

Catalog Number:

25-0042-82

Clone Number:

RM4-5

It is well known that B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I wanted to stain CD4 T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Wild-type mouse splenocytes

Primary Incubation

Surface staining with a-CD4 (1/200) (20 min, 4° in the dark)

Blocking Agent

Clone 2.4G2 (Fc block)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Yellow-green laser, flow cytometer

Results Summary

CD4 T cell can be stained

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

It works!

The Bad

N/A

The Bottom Line

It can be used.

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