Coomassie Fast and Easy to Use

Heidelberg University
Biochemistry
Graduate Student

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Quality of Results

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Company:

Carl Roth

Product Name:

ROTI Blue 1L Coomassie®-Staining solution

Catalog Number:

Art. No. A152.1

ROTI®Blue is a colloidal Coomassie® brilliant blue G250 staining agent for proteins in polyacrylamid. Hela cells WCL was load in SDS 4-12% precast gel, followed by staining with ROTI blue.

Experimental Design and Results Summary

Application

Polyacrilamyde gels staining.

Starting Material

Gel water methanol and ROTI blue 5x solution

Tips

No need to destain the gel after use, water can wash out the excess.

Results Summary

Hela cells WCL was loaded and a beads based purification was performed. Both WCL and eluate were loaded in 4-12% SDS gel and stained overnight. Water was used to destain the gel.for 15 ml of Roti blue solution: 5ml of ROTI blue, 5 ml of water and 5 ml of methanol were mixed

DOI or PMID #

N/A

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Summary

The Good

Precise staining<./p>

The Bad

The solution tends to precipitate forming corpuscles and particles. Mix well before aliquoting

The Bottom Line

Fast and easy to use product

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