Good Antibody for Clstn1

Sir William Dunn School of Pathology
Oxford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D systems

Product Name:

Human calsyntenin-1 antibody

Catalog Number:

AF5346

Clone Number:

N/A

To detect the protein level of Clstn1. The cells were lysed and cell lysates were tested by WB.

Experimental Design and Results Summary

Applications

Western Blot

Sample

RPE1 cell

Primary Incubation

1:100, 4 degree, overnight

Blocking Agent

1% milk

Secondary Incubation

1:5000, room temperature, 1 hour

Tertiary Incubation

no

Detection

ECL

Results Summary

This antibody could recognize both the full-length and the cleaved N-terminus of endognous Clstn1. In the Clstn1 knockdown sample, both bands clearly disappeared.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Can be used for detecting endogenous protein expression.

The Bad

Nothing

The Bottom Line

This is a good antibody for studying protein expression.

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