Boster Bio
Anti-phospho Lyn (Y396) Rabbit Monoclonal Antibody
P01424-1
Monoclonal IFI-12
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Staining of B cells from mice spleen after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with anti-phospho Lyn antibody from biobooster (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, secondary antibody from Invitrogen.
Flow Cytometry
B cells
0,25 µl/10^6 cells 50min RT
FBS 5%
F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0,1 µl/10^6
Nothing
Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody.
Easy to use
Waiting time for the delivery.
This product is really nice and work well by facs in b cells