Miltenyi Biotec
Ter-119 Antibody, anti-mouse, VioBlue®, REAfinity™
130-112-914
REA847
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) in health and disease, although in this particular experiment, we are looking to stain red blood cells that are Ter-119+.
Flow Cytometry
mouse spleen
anti-Ter119 VioBlue 1/50, 15' at 4°C
Fc block (2.4G2 clone)
NA
Flow cytometer
Comparing the same sample of mouse spleen before and after red blood cell lysis, clearly shows that we could stain red blood cells with this antibody.
It works.
It is not very bright.
It can be used.