Running Buffer for High Molecular Weight Protein Transfer

Johns Hopkins University School of Medicine
Department of Cell Biology
Graduate Student

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Company:

Bio-Rad

Product Name:

XT Tricine Running Buffer

Catalog Number:

1610790

This is 20x electrophoresis buffer for use with Criterion XT Tris-Acetate Gels. We use 3-8% Tris-Acetate Gels for high molecular weight protein (larger than 200kda) and we use this running buffer reagent.

Experimental Design and Results Summary

Application

Western blotting

Starting Material

Protein gel

Tips

This reagent is user friendly.

Results Summary

It is hard to do western blot for high molecular weight proteins. We used this running buffer and the results are clear. Only minimal background could be detected

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Summary

The Good

Reliable, high quality, user friendly

The Bad

None

The Bottom Line

I recommend this running buffer reagent for Immunoblotting of high molecular weight protein. It works very well with Bio-rad protein gels.

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