Phospho-Histone H2A.X (Ser139) Antibody that Works Great for Immunoblotting

July 01, 2020

Department of Cell Biology
Johns Hopkins University School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-Histone H2A.X (Ser139) Antibody

Catalog Number:

2577S

I have been using this antibody for two years to detect Phospho-Histone H2A.X in various cell lines using Immunoblotting. The antibody works great!

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines

Primary Incubation

1:1000 4 degrees overnight

Blocking Agent

5% BSA

Secondary Incubation

1:10000 RT 1h

Tertiary Incubation

N/A

Detection

LI-COR Odyssey CLx

Results Summary

There is one clear band at the expected molecular weight. Minimal background could be detected.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable, high quality, high specificity

The Bad

none

The Bottom Line

I highly recommend this antibody for Immunoblotting. It works very well.

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