Good Option to Stain Human CD4

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 510 anti-human CD4 Antibody

Catalog Number:

317444

Clone Number:

OKT4

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Occasionally, like in the present experiment, we stain T cells, in particular CD4 T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Healthy donor PBMCs depleted of CD8 T cells

Primary Incubation

anti-CD4 BV510 (1/200), 15', 4C

Blocking Agent

Fc Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

We could stain CD4 T cells.

Image Gallery

Summary

The Good

It works

The Bad

Could be brighter

The Bottom Line

It is a good option to stain human CD4

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