Mouse IgG HRP Antibody Is Great for Western Blotting

June 26, 2020

Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

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Company:

MilliporeSigma

Product Name:

Mouse IgG HRP Linked Whole Ab

Catalog Number:

GENA931-1ML

Browse Similar Products: IgG Western Blot Antibodies

The mouse IgG HRP antibody was used as secondary antibody for western blots, providing bands with little to no non-specificity. Samples tested were human breast cancer cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cells

Primary Incubation

Overnight

Blocking Agent

5% BSA

Secondary Incubation

1:5000

Tertiary Incubation

2 minutes with ECL

Detection

ECL Reagents

Results Summary

Clear specific bands were observed with incubation with the primary and mouse secondary antibody. It was comparable to other mouse secondary antibodies available.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clean specific bands

The Bad

N/A

The Bottom Line

This is a highly recommended secondary antibody for western blots.

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