Good Antibody for NEDD4L Western Blot

June 19, 2020

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Bethyl Laboratories Inc

Product Name:

NEDD4L Antibody

Catalog Number:

A302-512A

Clone Number:

Polyclonal

Browse Similar Products: NEDD4L Western Blot Antibodies

I use this antibody to detect endogenous NEDD4L by western blot from human cell lines.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell line

Primary Incubation

1:1000 4 ℃ overnight

Blocking Agent

5% milk

Secondary Incubation

1:2000 room temp 1 hour

Tertiary Incubation

None

Detection

ECL

Results Summary

I detect clear bands at expected molecular weight. They appeared to be doublet bands sometimes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong signal

The Bad

Nonspecific bands were also detected

The Bottom Line

I would recommend this antibody for NEDD4L as it works well to detect desired protein by western blot.

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