The Best NEDD4 Antibody I Have Tested - CST

June 25, 2020

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

NEDD4 Antibody

Catalog Number:

2740

Clone Number:

Polyclonal

I use this antibody to detect endogenous NEDD4 from different cell lines by western blot. This is the one that gave me best results.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell line

Primary Incubation

1:1000 4 ℃ overnight

Blocking Agent

5% milk

Secondary Incubation

1:2000 room temp 1 hour

Tertiary Incubation

None

Detection

ECL

Results Summary

Strong and specific bands were detected at the expected molecular weight. Background was clean.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong and specific signal

The Bad

None

The Bottom Line

I would recommended this antibody for NEDD4 western blot. The one from Santa Cruz also worked fine, but was discontinued.

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