Antibody for ITCH Working Well in Western Blot

June 18, 2020

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bethyl Laboratories Inc

Product Name:

Itch Antibody

Catalog Number:

A301-992A

Clone Number:

Polyclonal

Browse Similar Products: ITCH Western Blot Antibodies

I use this antibody to detect ITCH in western blot experiment. It works well with good and specific signal.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell line

Primary Incubation

1:1000 4 ℃ overnight

Blocking Agent

5% milk

Secondary Incubation

1:2000 room temp 1 hour

Tertiary Incubation

None

Detection

ECL

Results Summary

I obtained strong bands at the correct molecular weight. There could be some nonspecific bands depending on which cell line used.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong and specific signal

The Bad

Nonspecific bands were also detected sometimes

The Bottom Line

I would recommend this antibody for western blot analysis for ITCH endogenous expression in various cell lines.

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