PE Conjugated Anti-SSEA4 Antibody for Flow Cytometry

May 29, 2020

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-human SSEA-4 Antibody

Catalog Number:

330405

Clone Number:

MC-813-70

I used this antibody to confirm the pluripotency state of human embryonic stem cells by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

H9 cell line

Primary Incubation

1:50, room temperature, 15 minutes

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

This antibody produced good signal for embryonic stem cells. As the stem cells differentiated, the SSEA4 staining became weaker.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Strong and specific signal

The Bad

None

The Bottom Line

I would recommend using this antibody to stain embryonic stem cells as a marker.

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