Very Good Phospho-ERK Antibody for Mouse Lymphocytes

June 24, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370

Catalog Number:

4370

Clone Number:

D13.14.4E

We are interested in mTOR signaling and how it affects lymphocyte differentiation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymphocyte lysates

Primary Incubation

1:10000 in 4% BSA

Blocking Agent

5% milk

Secondary Incubation

1:5000 in 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

Very specific and strong signal for p-ERK.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very strong signal

The Bad

None

The Bottom Line

Just need to titrate the antibody for the cell type. It is definitely very specific and strong signal.

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