Great Antibody for Phospho-FOXO1/3

June 17, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb #2599

Catalog Number:

2599

Clone Number:

4G6

We are trying to understand signaling that feeds into T cell activation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymphocyte lysate

Primary Incubation

1:2500 in 4% BSA

Blocking Agent

5% milk

Secondary Incubation

1:5000 in 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

Able to detect upregulation of p-foxo1/3 upon TCR activation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good antibody

The Bad

None

The Bottom Line

Very good and sensitive antibody to detect p-Foxo1/3 in T cells.

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