Decent Id2 Antibody for Western Blot

June 10, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Id2 (D39E8) Rabbit mAb #3431

Catalog Number:

3431

Clone Number:

D39E8

We are interested in studying how metabolism regulates T cell activation and differentiation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymphocyte

Primary Incubation

1:1000 in 4% BSA

Blocking Agent

5% milk

Secondary Incubation

1:2500 in 5% milk

Tertiary Incubation

None

Detection

ECL solution

Results Summary

Able to detect upregulation of Id2 upon T cell activation. However, sometimes might need more sensitive ECL reagents to detect the protein.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clean band

The Bad

None

The Bottom Line

Great antibody, but might need to adjust concentration depending on Id2 expression for the cell type.

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