Great Clone for Granzyme B Expression

June 02, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-human/mouse Granzyme B Antibody

Catalog Number:

515406

Clone Number:

GB11

We are trying to study CD8 T cell differentiation in response to viral infection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

Fc block

Blocking Agent

FACS buffer

Secondary Incubation

Surface staining

Tertiary Incubation

ICS at RT for 1hr

Detection

Flow cytometry

Results Summary

Very bright staining from CD8 T cells upon ex vivo restim.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Consistently staining

The Bad

None

The Bottom Line

The clone and the fluorochrome is really good at detecting granzyme B.

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