Great Antibody to Detect CD4 T Cells Via Flow Cytometry

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD4 Monoclonal Antibody-Alexa Fluor 532

Catalog Number:

58-0042-82

Clone Number:

(RM4-5)

We are an immunology lab that looks at immune responses in the context of inflammation and cancer. This marker is used to widely used to identify CD4 T cells in flow cytometry experiments.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes (Naive and Infected)

Primary Incubation

Multiple Dilutions (1:250, 500, 1000)

Blocking Agent

FC Block 100ng/mL in FACS solution (surface)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Cytek Aurora (BVR)

Results Summary

Splenic CD4 T cells cells are strongly and specifically labelled to identify the population with CD4 AF532

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable results as easy to idenitify CD4+ T cells from other spenic cells.

The Bad

None

The Bottom Line

I would recommend this product to anyone. It gives a strong, consistent labeling of CD4 T cells.

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