Background Buster Reduces Overall Background for IHC

May 13, 2020

UAB
Radiation Oncology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Innovex Biosciences

Product Name:

Background Buster

Catalog Number:

NB306-50

Background Buster was used to minimize background staining and hence elevate target protein staining. For IHC, as background staining is often encountered, it is important to use such a reagent.

Experimental Design and Results Summary

Application

Immunohistochemistry (Immunofluorescence)

Starting Material

Mouse breast tumor tissue

Tips

Use a pipet to reduce waste of background buster. Only amount to cover sample is enough, so dropper is not necessary.

Results Summary

Background staining was not detected and target protein staining was apparent under microscope for organoid specimens.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Very easy to use

The Bad

N/A

The Bottom Line

The Background Buster reagent should be used to reduce background staining and is highly recommended for any IHC procedures. The reagent should be used following treatment of specimens with 3% hydrogen peroxide.

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