This BioLegend Antibody Stains CD45.2+ Cells Very Well

June 02, 2020

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Dazzle™ 594 anti-mouse CD45.2 Antibody

Catalog Number:

109846

Clone Number:

104

We wanted to study immune cells in the disease vs healthy state, so we used the antibody to identify CD45.2+ cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of tumor cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody is great for identifying the immune cells in the tissue.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody is great for identifying CD45.2+ cells

The Bad

None to mention

The Bottom Line

The antibody is very useful for distinguishing the immune cell population in the tissue.

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