This BioLegend Antibody Stains Ki67+ Cells Very Well

May 27, 2020

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-mouse Ki-67 Antibody

Catalog Number:

652420

Clone Number:

16A8

We wanted to assess cell proliferation in the disease vs healthy state, so we used the antibody to stain for Ki67+ proliferating cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of tumor cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stained proliferating populations very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody is great for identifying proliferating cells

The Bad

None to mention

The Bottom Line

The antibody is good for detecting Ki67+ proliferating cells.

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