This BioLegend Antibody Stains PD-L2+ Cells Very Well

May 15, 2020

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD273 (B7-DC, PD-L2) Antibody

Catalog Number:

107219

Clone Number:

TY25

We wanted to study PD-L2+ cells in the disease vs healthy state, so we used the antibody to identify PD-L2+ cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of tumor cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody clearly identified PD-L2+ cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody clearly stains PD-L2+ cells

The Bad

None to mention

The Bottom Line

The antibody is great for identifying PD-L2+ cells in the tissue.

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