This BioLegend Antibody Stains TNFa Very Well

April 27, 2020

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse TNF-α Antibody

Catalog Number:

506328

Clone Number:

MP6-XT22

We wanted to examine differences in TNFa secretion in two different conditions, so we used this antibody to stain for TNFa.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody made the TNFa+ population very clear and distinguishable

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody clearly identifies TNFa+ cells

The Bad

None to mention

The Bottom Line

The antibody is great for identifying TNFa-secreting cells

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