Measuring Expression of CD51 on Cultured Mouse Splenocytes

April 23, 2021

microbiology
NYU
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse CD51 Antibody

Catalog Number:

104105

Clone Number:

RMV-7

We are interested In detection of alpha V integrin or CD51 on cultured T helper cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cultured mouse TH0 cells

Primary Incubation

1:200, room temp, 30 min

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

ZE5 yeti flow cytometer

Results Summary

Alpha V integrin was detected on cultured TH0 cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright, repeatable, was validated in tissues.

The Bad

Unable to distinguish distinctive populations.

The Bottom Line

Works well and reliable

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