CD5 Antibody Gives Great Results

April 16, 2021

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-mouse CD5 Antibody

Catalog Number:

100605

Clone Number:

53-7.3

We are interested in detection of B1a and B1b cells in mouse spleen

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200, 30 minutes, room temp

Blocking Agent

FC block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

ZE5 Biorad flow cytometer

Results Summary

By gating on CD19+ B220- cells were were able to identify CD5+ B1a cells and CD5- B1b cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright, easy to use

The Bad

N/A

The Bottom Line

Reliable and repeatable

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