Good Golgi Marker; However, Lower Resolution than Other Markers

May 15, 2020

Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

RCAS1 (D2B6N) XP® Rabbit mAb

Catalog Number:

12290

Clone Number:

D2B6N

This was tested and compared to Glg1 which showed slightly better staining of the golgi, however this antibody also produced an excellent signal.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

MDA-MB-231 BrCa

Primary Incubation

1:500

Blocking Agent

5% goat serum

Secondary Incubation

1:2000 Al647

Tertiary Incubation

None

Detection

Confocal Microscopy

Results Summary

Results showed good staining of the golgi at 1:500 with minimal background. Anti-Glg1 showed slightly broader staining however.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good golgi stain in cancer cells with no background

The Bad

Other golgi markers showed slightly more resolution and broader staining

The Bottom Line

Excellent marker for the golgi when used at 1:500, didn't require any additional titration.

Share your experience with other scientists. Write a Review! »

Join the discussion