Detection of CCR6+ CD4 T Cells

March 03, 2021

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse CD196 (CCR6) Antibody

Catalog Number:

129803

Clone Number:

29-2L17

We are interested in detecting CCR6+ CD4 T cells in the spleens of mice

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200, room temp, 20 min

Blocking Agent

FC block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

ZE5 Yeti flow cytometer

Results Summary

We were able to detect CCR6high + CD4 T cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright, able to distinguish between a CCR6 high population

The Bad

N/A

The Bottom Line

repeatable and reliable

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