Abcam
Anti-alpha Tubulin antibody [DM1A] - Loading Control
ab7291
DM1A
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This tubulin antibody is very useful as loading control. Even after several times of stripping, this antibody still shows nice tubulin band.
Western Blot
HEK293T, COS7, HCT116, HaLa cell lines
1:3000 dilution, 1h at RT
5% non-fat milk in TBST, 30 min at RT
Anti-mouse IRDye680
N/A. The blots were immediately checked after wash.
Odyssey and image studio for quantification
This antibody is very sensitive that 1hour incubation at room temperature gives sufficient intensity even after multiple times of membrane stripping. I prefer using this to an actin antibody.
N/A
Sensitivity and specificity
Nothing
A useful antibody to have