Detection of Mouse Germinal Center B Cells

May 08, 2020

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cyanine5.5 anti-mouse CD95 (Fas) Antibody

Catalog Number:

152609

Clone Number:

SA367H8

Mice were exposed to antigen and germinal center B cells were detected in draining lymph nodes

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph node cells

Primary Incubation

1:200, 20 min, room temperature

Blocking Agent

FC block

Secondary Incubation

-

Tertiary Incubation

-

Detection

ZE5 YETI Bio-Rad flow cytometer

Results Summary

GC B cells were detected in mice exposed to antigen (left) or left unchallenged (Right)

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, reliable

The Bad

-

The Bottom Line

Repeatable

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