Detection of SiglecF+ Cells in Mice

May 06, 2020

microbiology
NYU
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD170 (Siglec-F) Antibody

Catalog Number:

155505

Clone Number:

S17007L

Mice were sensitized with A fumigatus and SiglecF+ cells were detected in the lung

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung cells

Primary Incubation

1:200, 20 minutes, room temperature

Blocking Agent

FC block

Secondary Incubation

-

Tertiary Incubation

-

Detection

ZE5 YETI Biolegend flow cytometer

Results Summary

SiglecF+ CD11c+ or CD11c- cells were detectable in the lungs of mice sensitized with A. fumigatus

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright antibody, easy to use

The Bad

-

The Bottom Line

Easy to use and reliable.

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