Cell Signaling Technology
NEDD8 Rabbit
2745S
Polyclonal
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Browse Similar Products: NEDD8 Western Blot Antibodies
This antibody has many bands that I am not sure whether it is specific or not. When cells are treated with NAE1 inhibitor (MLN4924), the intensity of many bands does not go down, so, it is likely that these bands are non-specific.
Western Blot
HEK293T cell lysate
1:2000, 4C, overnight.
5% non-fat milk in TBST, 30 min at room temperature
Rabbit IRDye-800 for quantitative western
N/A
Odyssey and image studio software
I can definitely see Cullins conjugated to Nedd8, but there are many other bands..
Okay sensitivity
Off-targets
Good for rough validation of NEDD8-conjugation level in cells.