Cell Signaling Technology
Anti-PERK
5683
D11A8
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Browse Similar Products: PERK Western Blot Antibodies
This rabbit monoclonal antibody worked well on HeLa cell lysates.
Western Blot
HeLa cells ±5microgram/ml tunicamycin or 100mM thapsigargin for 4 hours
1:5000 in 5% milk in TBST
5% milk in TBST
1:20000 goat anti-rabbit HRP (Biorad #1706515)
Not applicable
Clarity max ECL substrate (Biorad #1705062)
This antibody was used to detect PERK in HeLa cells ± ER-stress. The antibody worked well with minimal cross-reactivity with a smaller molecular weight protein.
N/A
Works well for western blotting
Have not tried it with other applications
This rabbit monoclonal antibody detects PERK in HeLa cells. There is minimal non-specific binding around 100kDa.