Detection of CD4 T Cells in Mouse Tissues

April 21, 2020

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD4 Antibody

Catalog Number:

100443

Clone Number:

GK1.5

We are interested in detecting CD4 T cells in mouse tissues.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200, 20 min, room temperature

Blocking Agent

FC block

Secondary Incubation

-

Tertiary Incubation

-

Detection

Ze5 YETI Biorad flow cytometer

Results Summary

CD4 and CD8 T cells identified in mouse splenocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright, easy to use, clean detection

The Bad

-

The Bottom Line

Compatible with the use of many different antibodies in a panel. Clean and great detection.

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