Detection of IL-17A Producing Cells

April 20, 2020

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse IL-17A Antibody

Catalog Number:

506904

Clone Number:

TC11-18H10.1

We are interested in detecting IL-17A-producing cells in infected or autoimmune animals.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph node cells

Primary Incubation

1:200, 30 minutes, room temperature

Blocking Agent

FC block

Secondary Incubation

-

Tertiary Incubation

-

Detection

ZE5 Biorad yeti flow cytometer

Results Summary

Lymphocytes from infected mice were restimulated and intracellular production of IFNG and IL-17A was detected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Can be used with anti-IFNG

The Bad

Sometimes to bright

The Bottom Line

Easy and reliable detection of IL-17A.

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