Detection of CD45+ Cells in Mouse Tissues

April 10, 2020

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605™ anti-mouse CD45 Antibody

Catalog Number:

103155

Clone Number:

30-F11

We are interested in detection of CD45+ CD4+ T cells in the spinal cord of mice with experimental autoimmune encephalomyelitis.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spinal cord

Primary Incubation

1:200, 30 min, room temp

Blocking Agent

FC Block

Secondary Incubation

-

Tertiary Incubation

-

Detection

ZE5 yeti Biorad flow cytometer

Results Summary

Spinal cords from mice with EAE (left graph) and without EAE (right) were digested, and CD4+ CD45+ T cells were identified by flow cytometery

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easily detectable

The Bad

-

The Bottom Line

Very bright and reliable antibody.

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