BioLegend
PE/Cy7 anti-mouse TCR Vβ5.1, 5.2 Antibody
139508
MR9-4
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Transferred OT2 T cells were detected using antibody to TCR Vβ5
Flow Cytometry
Mouse spleen
1:200, 20 minutes, room temperature
FC Block
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ZE5 YETI Biorad Flow cytometer
CD45.2 OT2 T cells were transferred to CD45.1 mice. OT2 T cells were detected using TCR Vβ5 antibody and confirmed with CD45.2 distinction.
N/A
Very good separation, reliable, repeatable, can use with CD45.1 for CD45.2 OT2 T cells
Have to use with CD45.1 or CD45.2 to distinguish small number of host TCR Vβ5 cells
Easy to use with good separation.