Effectively Lowers Glycolytic Activity at 5 uM

Princeton
Molecular Biology
Graduate Student

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Company:

Tocris

Product Name:

2-Deoxy-D-glucose

Catalog Number:

4515

This was used at 5 mM overnight to competitively inhibit glycolysis in a basal media with 4.5 g glucose/L. Results showed effective inhibition.

Experimental Design and Results Summary

Application

Glycolysis monitoring by flow cytometry

Starting Material

Cancer cell lines in culture

Tips

Used at 5 mM, however a larger supply is needed than 50 mg as this amount goes by quickly

Results Summary

Results showed 30-50% inhibition of glycolytic activity in cells treated with 5mM. Some cell lines died at this level, while others were fine.

DOI or PMID #

N/A

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Summary

The Good

Easy to dissolve to 500 mM in PBS.

The Bad

Larger order sizes needed than 50 mg

The Bottom Line

Good price for 2DG between all the vendors. Works as expected.

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