BioLegend
PE anti-mouse CXCR5
145504
L138D7
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This antibody is a repeatable antibody for identification of TFH cells (CXCR5+ PD1+) from lymph nodes and spleenocytes of immunized or infected mice
Flow Cytometry
Mouse spleenocytes (immunized)
1:50, room temp, 30 min
None
Flow cytometry
Splenocytes from mice that were and were not immunized were stained and TFH cells were identified as CD4+ cells that are also CXCR5+ and PD-1+
N/A
Other colors in this clone don't work as well
Repeatable, population is easy to detect, no secondary
Have to use at 1:50
The best antibody to CXCR5 that we were able to find.