Expression of JNK in Microglia Cells

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Company:

Cell Signaling Technology

Product Name:

SAPK/JNK Antibody

Catalog Number:

9252

Clone Number:

Polyclonal

BV2 microglia cells were treated with 1µM LPA in the presence or absence of LPAR5 inhibitor. Changes in the expression of JNK were detected using immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 Microglia cells

Primary Incubation

1:1000, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL

Results Summary

No changes were observed in the expression of p38 between different samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works very well

The Bad

N/A

The Bottom Line

Great antibody for the detection of p38.

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