Expression of pJNK in Microglia Cells

April 08, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Company:

Cell Signaling Technology

Product Name:

Phospho-SAPK/JNK (Thr183/Tyr185) Antibody

Catalog Number:

9251

Clone Number:

Polyclonal

BV2 microglia cells were treated with 1µM LPA in the presence or absence of LPAR5 inhibitor. Changes in the phosphorylation of JNK were detected using immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 Microglia cells

Primary Incubation

1:1000, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL plus

Results Summary

Treatment with 1µM LPA increased the expression of pJNK in BV2 cells. This effect was abrogated in the presence of LPAR5 inhibitor (TCLPA5).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works as expected

The Bad

Some times the bands appear to be not so strong and needs longer exposure time

The Bottom Line

Generally a good and reliable antibody.

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