Expression of pERK1/2 in Microglia Cells

April 02, 2020

Molecular Biology and Biochemistry
Med Uni Graz
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Company:

Cell Signaling Technology

Product Name:

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody

Catalog Number:

9101

Clone Number:

Polyclonal

BV2 microglia cells were treated with 1µM LPA in the presence or absence of LPAR5 inhibitor. The inhibitor was tested for its efficacy and changes in its effect after different pre-incubation periods. Changes in the expression of pERK were detected using immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 Microglia cells

Primary Incubation

1:1000, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL plus

Results Summary

Treatment with 1µM LPA increased the expression of pERK1/2 in BV2 cells. This effect was abrogated in the presence of LPAR5 inhibitor (TCLPA5).

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Works very well

The Bad

N/A

The Bottom Line

A reliable and broadly used Ab.

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