ß-Tubulin Antibody Used as a Loading Control for Immunoblotting

March 27, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Company:

MilliporeSigma

Product Name:

Monoclonal Anti-β-Tubulin antibody produced in mouse

Catalog Number:

T4026

Clone Number:

Clone TUB 2.1

BV2 microglia were treated with LPA and LPA plus an LPAR5 inhibitor for different time periods. The expression of different proteins was analysed and β-Tubulin was used as a loading control.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 Microglia cells

Primary Incubation

1:500, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti mouse

Tertiary Incubation

N/A

Detection

ECL

Results Summary

No changes were observed in the expression of β-Tubulin (as expected).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works very well

The Bad

N/A

The Bottom Line

Excellent loading control for immunoblotting experiments.

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