Changes in the Expression of GLUT1 in Microglia Cells

March 06, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Company:

Abcam

Product Name:

Anti-Glucose Transporter GLUT1 antibody [SPM498]

Catalog Number:

ab40084

Clone Number:

SPM498

Primary murine microglia were treated with LPA for different time periods. The purpose of the experiment was to detect possible changes in the expression of GLUT1 in microglia cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary murine microglia cells

Primary Incubation

1:500, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti mouse

Tertiary Incubation

N/A

Detection

ECL plus

Results Summary

Changes were observed in the expression of GLUT1 in microglia cells after LPA treatment for 2 and 8 hours.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works quite well

The Bad

N/A

The Bottom Line

A good antibody for detecting changes in GLUT1 expression in microglia.

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