Great Smooth Muscle Actin Antibody

Neuroscience
Wisconsin University
Postdoctoral Researcher

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Company:

Novus Biologicals

Product Name:

Alpha-Smooth Muscle Actin Antibody (1A4/asm-1)

Catalog Number:

NBP2-33006

Clone Number:

1A4

Our research focuses on understanding multifactorial pathological aspects in neurodegenerative diseases. The specific goal of this experiment was to evaluate the vascular morphology in the course of disease progression. This antibody demonstrated excellent staining for perivascular smooth muscle cells as well as pericytes.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Feline eye

Primary Incubation

Diluted at 1:800, overnight at 4°C

Blocking Agent

10% normal donkey serum + 0.5% triton X + 0.1M PBS

Secondary Incubation

Alexa 488 conjugated anti-mouse IgG 1:500, 1 hr at RT

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Robust signals from the immunolabelling for smooth muscle actin was detected in feline ocular tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Highly specific with no background

The Bad

N/A

The Bottom Line

Very sensitive perivascular smooth muscle cells markers for feline species and generates robust signals (red).

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