Invitrogen ZO-1 Antibody that Works Really Well

January 22, 2020

Genetics
Emory University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

ZO-1 Monoclonal Antibody

Catalog Number:

33-9100

Clone Number:

ZO1-1A12

We use this antibody to stain for neural rosettes in our cortical organoid sections and the stains come out beautifully. We use it at a dilution of 1:100.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Organoid

Primary Incubation

1:100, overnight, 4C

Blocking Agent

0.3% Triton X-100 & 10% Donkey Serum in PBS

Secondary Incubation

1:1000 anti-mouse 594

Tertiary Incubation

N/A

Detection

Fluorescent Microscope

Results Summary

We get beautiful stains of our organoid sections that we can image.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works very well

The Bad

Requires a lot of antibody

The Bottom Line

Pricey and need a lot, but produces beautiful stains.

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