Great CD279 (PD-1) Monoclonal Antibody

January 30, 2020

Innate Immunity
DRFZ
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™

Product Name:

CD279 (PD-1) Monoclonal Antibody (J43), FITC

Catalog Number:

11-9985-82

Clone Number:

J43

We used this antibody to identify potential PD-1+ populations within TCRbeta+ population of murine bone marrow. The antibody is good with high resolution.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine bone marrow cells

Primary Incubation

20mins 37oC

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

Good, high resolution, could identify PD1+ cells in murine bone marrow.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good, sensitive, high resolution

The Bad

No bad things about it

The Bottom Line

I would recommend this antibody

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