Great Antibody for Detecting Phosphorylation Levels of MAPK

March 27, 2020

IMCB
NTHU
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)

Catalog Number:

4370

Clone Number:

D13.14.4E

The cells are subjected to various conditions and they changes in the phosphorylation levels of MAPK is detected using western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine Myeloid Cells

Primary Incubation

1:1000, O/N, 4 degree C

Blocking Agent

1% BSA

Secondary Incubation

1:10000, 1hr, RTP

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

The bands are sharp and clear.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy and sensitive

The Bad

N/A

The Bottom Line

Highly recommended to those who wishes to detect the levels of phosphorylation of MAPK.

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