Easy-to-Use Antibody for the Detection of HIF1 Alpha

March 03, 2020

IMCB
NTHU
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

HIF1 alpha

Catalog Number:

GTX127309

Clone Number:

Polyclonal

The cells are subjected to various conditions to test for the expression of HIF1 alpha as an indicator of induction of hypoxic condition.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lines

Primary Incubation

1:1000, O/N, 4 degree C

Blocking Agent

3% Milk

Secondary Incubation

1:10000, 1hr, RTP

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

The bands are clear and defined. It is easily detected.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use and sensitive

The Bad

NIL

The Bottom Line

A good antibody that is recommended to those detecting HIF1 alpha.

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