Abcam
ATXN7L3 Antibody
ab99947
Polyclonal
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ATXN7L3 is member of the SAGA DUBm complex that is responsible for the acetylation and deubiquitination of chromatin. Here we assess the expression of ATXN7L3 in acute myeloid leukemia cell line OCI AML-2.
Western Blot
AML2 whole cell lysate
1:1000 RT 1hr in 5% skim milk
5% milk - 10 min room temperature
5% milk - 30 min room temperature secondary Ab 1:10K
N/A
ECL film
Good antibody for western blot with low background. Approx. size 40 kDa. Conditions represent samples following CRISPR/Cas9 deletion of ATNX7L3 using two different sgRNAs. sgLacZ represents non-targeting control. GAPDH is shown as the loading control.
Antibody worked well.
Non-specific band just below ATXN7L3
Antibody works well.