Great Antibody for Tubulin

January 28, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Monoclonal Anti-β-Tubulin antibody produced in mouse

Catalog Number:

T8328

Clone Number:

AA2

We are interested in studying the mechanism of T cell differentiation through modulating mTOR pathway.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse lymphocyte

Primary Incubation

1:1000

Blocking Agent

10% goat serum

Secondary Incubation

Anti-mouse AF488

Tertiary Incubation

None

Detection

Confocal Microscopy

Results Summary

Very nice cytoskeletal staining, especially in dividing cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very nice staining and very specific. Minimal background staining

The Bad

None

The Bottom Line

Very useful antibody for both IF and WB.

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